The Ultimate Guide to BPC 157 and TB 500 Dosage for Healing

Are you looking to enhance your body’s natural healing process, optimize overall wellness, or explore advanced tools for peak performance? In this guide, we’ll dive into two of the most talked-about peptides in the biohacker and performance community: BPC 157 and TB-500.

You’ll learn how they work, why stacking them creates a synergistic effect, and what kind of results you can expect when dosing them effectively. From tissue repair and gut health to faster recovery and performance optimization, this guide will give you a clear, practical look at what makes this peptide duo so powerful.

Introduction to BPC 157 and TB-500 Dosage

On their own, both peptides are powerful. But when combined, they deliver a synergistic effect—BPC 157 helping with tissue protection and repair, while TB-500 improves circulation and cell migration into damaged areas. Together, they act like an accelerator for recovery and resilience, giving your body the tools it needs to bounce back stronger.

Because of this versatility, the stack has found a place not just in injury rehab, but also in performance optimization, gut health support, and whole-body wellness strategies. Whether your goal is to push training boundaries, reduce downtime, or simply feel better day-to-day, the right dosage protocol can make all the difference.

A typical starting point for this stack is 250–500 mcg of each peptide per day, usually run in 6–8 week cycles. After that, it’s smart to take the same amount of time off to let your system reset before starting again. You can get them in a combination vial where both peptides are blended together 1:1 to make dosing more simple.

If you are new to peptides be sure to read our article Peptide Safety 101: How to Reconstitute, Inject, and Store Peptides the Right Way.

Overview of BPC 157 and TB-500 Blend

The BPC 157 + TB-500 blend has gained popularity among athletes, biohackers, and wellness enthusiasts for its versatility. Rather than targeting just one issue, this stack supports multiple areas of recovery and performance—making it a go-to for anyone who wants a more comprehensive approach to healing and optimization.

People often turn to this blend for:

• Muscle and tendon recovery after strenuous training or injury

• Joint health and mobility support

• Gut repair and systemic inflammation control

• Overall performance—feeling stronger, more resilient, and able to bounce back faster

Because it covers so many bases, the blend has become known as the “Wolverine protocol”— speeding up recovery and keeping the body in fighting shape. It’s not just about repairing damage—it’s also about building a stronger foundation for long-term wellness and performance.

Understanding BPC 157

BPC 157 is a peptide derived from a protective protein in the stomach, widely recognized for its role in tissue repair, gut health, and inflammation control.

Its key strength lies in boosting collagen production, which helps tissues heal more effectively (this goes for muscles, tendons, ligaments, and skin). It also supports angiogenesis—the growth of new blood vessels—ensuring nutrients and oxygen reach damaged areas more efficiently.

In practice, BPC 157 is often associated with:

• Faster recovery from muscle and tendon strain

• Reduced joint inflammation and discomfort

• Gut lining repair and improved digestion

• Broader protective effects on the nervous system

Thanks to its versatility, BPC 157 is often considered a foundational peptide for both recovery and overall wellness.

A typical dosage range is 250–500 mcg daily for 4–8 weeks at a time.

For a deeper dive into working with this peptide, read our full report BPC-157: How it Works, Benefits, Dosage, Side Effects and Safety.

Understanding TB-500

TB-500 is a synthetic peptide based on a natural protein fragment called thymosin beta-4, which plays a key role in cell movement and tissue repair. Unlike BPC 157, which is often linked to protecting and repairing tissue, TB-500 is more about mobilizing the body’s repair machinery—getting cells where they’re needed most.

It’s especially valued for:

• Soft tissue healing — muscles, tendons, and ligaments

• Improved flexibility and mobility — reducing stiffness and supporting joint health

• Enhanced circulation — delivering nutrients and oxygen to damaged areas more efficiently

This makes TB-500 a favorite among athletes, high performers, and anyone who wants to recover faster and move more freely.

Most people work with a dosage of 250–500 mcg daily for a 4–8 week cycle.

For a deeper dive into working with this peptide, read our full report TB-500: How it Works, Benefits, Dosage, Side Effects and Safety.

Mechanism of Action

TB-500 works by guiding repair where it’s needed most. It stimulates cell migration—helping healing cells travel quickly to damaged tissue—and supports angiogenesis, or new blood vessel growth. Together, these actions improve circulation and deliver nutrients more efficiently, creating the conditions for faster recovery and greater mobility.

Benefits of BPC 157 and TB-500

BPC 157 and TB-500 complement each other in a very powerful way. While each peptide has its own strengths, together they create a multi-layered repair system that supports faster recovery and greater resilience.

• BPC 157 promotes collagen production, protects tissues from further damage, and regulates inflammation at the source.

• TB-500 enhances cell migration and stimulates angiogenesis (the growth of new blood vessels), ensuring that nutrients and oxygen flow efficiently to areas under stress.

By working in tandem, they:

• Boost tissue remodeling and structural repair

• Improve blood flow and nutrient delivery

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• Keep inflammation in check while accelerating regeneration

This synergy is what makes the stack so effective—BPC 157 lays the groundwork for healing, and TB-500 powers the delivery system that makes the process faster and more efficient.

Results of BPC 157 and TB-500 Dosage

When dosed properly, the BPC 157 + TB-500 stack produces noticeable results across different situations. The key is knowing how much to take, for how long, and how to prepare your vials the right way.

What to Expect

If you’re consistent with your dosing, most people begin noticing effects within 1–2 weeks—including reduced pain, faster recovery, and improved mobility. Gut-related improvements may take longer, often becoming more noticeable by week 3 or 4. Full results typically build over the course of a 4–8 week cycle.

Use a daily subcutaneous dose of 250–500 mcg of BPC-157 and 250–500 mcg of TB-500, delivered with a 1ml insulin syringe or peptide pen. We recommend sourcing from BioEdge Research Labs—a trusted supplier that offers exclusively third-party tested, >99% pure peptides manufactured in cGMP-certified facilities. Their TB-500 + BPC-157 combination vial contains both peptides in a 1:1 ratio making dosage simple and precise.

Here’s how to adjust your protocol based on your goals:

Injury Recovery (Muscle, Tendon, Ligament)

• Dose: 250–500 mcg of each peptide, once daily
• Injection site: Near the injury if possible
• Cycle: 4–6 weeks for acute injuries; up to 8 weeks for chronic recovery

Joint Pain & Inflammation

• Dose: 300–500 mcg of each peptide daily
• Injection site: Rotate between sites near affected joints or lower abdomen
• Cycle: 6–8 weeks

Gut Health & Systemic Inflammation

• Dose: 250–400 mcg of each peptide daily
• Injection site: Subcutaneous abdominal fat is ideal
• Cycle: 4–6 weeks

Full-Body Recovery & Performance Support

• Dose: 500 mcg of each peptide per day
• Injection site: Rotate daily for even absorption (abdomen, thigh, upper glute)
• Cycle: 6–8 weeks on, followed by 6–8 weeks off

Reconstitution & Delivery Tips for BPC-157 + TB-500

To prepare your peptides, reconstitute your 10mg vial (blended or separate) with 2mL of reconstitution solution aka bacteriostatic water. Add the water slowly down the side of the vial—don’t shake. Once mixed, store in the fridge and use within 6–8 weeks.

For daily dosing, you can use a 1ml insulin syringe or a peptide pen. If you’re using a pen, make sure it can deliver at least 0.1 mL per dose—some pens only deliver 0.05 mL per click, so confirm your model’s capacity before purchasing.

Daily Dose Volume Reference:

• For 250 mcg, draw or dial to 0.05 mL (5 units on an insulin syringe)
• For 500 mcg, draw or dial to 0.1 mL (10 units)

*If you want to see how that math works – here it is. Based on reconstituting 10mg into 2mL you get 10,000 mcg of peptide per 2mL which equals 5,000 mcg per 1mL. So a 500 mcg dose divided by this 5,000mcg in the 1mL of solution equals 0.1 mL; or for a 250 mcg dose ÷ 5,000 = 0.05 mL.

These doses apply whether you’re using a blended vial, or drawing equal parts from separate vials into one syringe.

Again, if you are new to peptide therapy we recommend reading Peptide Safety 101: How to Reconstitute, Inject, and Store Peptides the Right Way

Commonly Asked Questions about BPC 157 and TB-500

Are there side effects?
Both peptides are generally well-tolerated. Mild side effects can include temporary redness at the injection site, headache, or slight fatigue. These usually pass quickly. Staying hydrated, rotating injection sites, and keeping doses in the recommended range helps minimize issues.

Are BPC 157 and TB-500 legal?
They are legal to buy and use for research purposes, and preclinical trials have shown amazingly consistent results. Many peptides including Semaglutide, Tirzepetide, and Tesamorelin are already used as prescription medicines.

Since most peptides like BPC-157 and TB-500 aren’t regulated by the FDA as drugs or supplements, it’s up to you to make sure you’re sourcing them responsibly. Don’t put your health at risk. We always recommend choosing suppliers that manufacture in a GMP-certified facility and publish real lab results—including HPLC, mass spectrometry, and heavy metals screening—so you can verify both purity and weight before you buy.

What’s the best way to inject?
You can use a fine insulin syringe or a peptide pen to deliver a subcutaneous injection into soft tissue, usually in the lower abdomen or thigh. Both methods are effective. No matter which you choose, be sure to rotate injection sites to reduce irritation and keep absorption steady.

Can I take them together in one shot?
Yes. If you’re using a blended vial, both peptides are already combined. If you’re using separate vials, you can reconstitute and draw each into one syringe.

How long until I notice results?

• Injury or joint pain: noticeable changes often within 1–2 weeks

• Gut health: improvements typically show up by week 3 or 4

• Performance & recovery: quicker turnaround between workouts within 10–14 days

Do I need to cycle off?
Yes. A standard cycle is 6–8 weeks on, followed by 6–8 weeks off. This prevents the body from becoming dependent and helps maintain effectiveness over time.

What about stacking with other peptides?
If you are dealing with repeated issues, slower healing, or who want anti-aging benefits along with recovery. GHK-Cu adds support for skin, collagen, and vascular repair.

If you’ve had surgery, major tendon tears, or are older and not recovering like you used to consider adding CJC/Ipamorelin to stimulate GH and IGF-1 for cellular growth and tissue regeneration. This stack with is built for serious recovery demands: multiple injuries, deep inflammation, or post-surgical healing.

If you do stack, introduce one peptide at a time so you can clearly track how your body responds.

Summary of BPC 157 and TB-500 Blend Review

The BPC 157 + TB-500 stack has earned its reputation as one of the most effective peptide pairings for healing and recovery. By combining BPC 157’s ability to protect and repair tissue with TB-500’s role in improving circulation and mobilizing repair cells, the blend creates a powerful foundation for recovery, resilience, and performance.

Most people see the best results with daily subcutaneous doses of 250–500 mcg of each peptide, run in 6–8 week cycles with equal time off. When prepared and measured correctly, this protocol supports faster tissue repair, reduced inflammation, better mobility, and gut health improvements.

References

TB-500 Tendon Healing and Fibroblast Migration

• Lee, J.Y., et al. (2013). Thymosin Beta 4 enhances the healing of medial collateral ligament in rat. Am J Sports Med, 41(7), 1440–1447. https://pubmed.ncbi.nlm.nih.gov/23523891

• Philp, D., et al. (2010). Thymosin Beta 4 enhances repair by organizing connective tissue in skin wound healing. J Invest Dermatol, 130(1), 287–294. https://pubmed.ncbi.nlm.nih.gov/20536458

TB-500 Inflammation Modulation

• Li, J., et al. (2018). Thymosin Beta 4 prevents oxidative stress, inflammation, and fibrosis in ethanol- and LPS-induced liver injury. Oxid Med Cell Longev, 2018, Article ID 9637560. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6079392

• Sosne, G., et al. (2018). Thymosin Beta 4 limits inflammation through autophagy. Curr Pharm Des, 24(30), 3540–3547. https://pubmed.ncbi.nlm.nih.gov/30063848

TB-500 in Gut Health and GI Repair

• Tomaselli, G., et al(2011). Immunoreactivity of Thymosin Beta 4 in human gastrointestinal tract. Eur J Histochem, 55(3), e29. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3167329

• Goldstein, A.L., et al. (2012). Thymosin Beta 4: A multifunctional regenerative peptide. Expert Opin Biol Ther, 12(1), 37–51. https://pubmed.ncbi.nlm.nih.gov/22074294

BPC-157 Wound Healing and Granulation Tissue Formation

• Sikiric, P., Seiwerth, S., & Staresinic, M. (1993). The effect of BPC 157 on granulation tissue formation, angiogenesis, and collagen production in rat skin wounds. Journal of Physiology and Pharmacology, 44(4), 413–421. https://pubmed.ncbi.nlm.nih.gov/9403790/
• Zoric, Z., et al. (2015). Body protective compound-157 enhances alkali-burn wound healing in vivo and promotes proliferation, migration, and angiogenesis in vitro. Journal of Surgical Research, 194(2), 469–477. https://doi.org/10.1016/j.jss.2014.10.015​

BPC-157 Tissue Regeneration in Muscle and Bone Healing

• Gwyer, D., Wragg, N. M., & Wilson, S. L. (2019). Gastric pentadecapeptide BPC 157 and its role in accelerating musculoskeletal soft tissue healing. Cell and Tissue Research, 377(2), 153–159. https://doi.org/10.1007/s00441-019-03016-8