A fixed example of 5 mg three times weekly for 10–12 weeks, with concentration calculations and a clear distinction between the example and published research.
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This MOTS-c dosage guide explains Joe Mars’s example protocol and the concentration math used to read a vial label. It separates that example from published research. It is not a prescribing guide.
5 mg per administration, three times a week, for 10–12 weeks. The amount stays fixed throughout the example. It is not a daily schedule.
| Part of the example | Amount or schedule |
|---|---|
| Amount each time | 5 mg (5,000 mcg) |
| Frequency | Three times per week |
| Duration | 10–12 weeks |
| Total per week | 15 mg |
| Changes during the example | The same 5 mg amount throughout |
This is the publisher’s example protocol, not a regimen established by a controlled human trial. The cited studies below do not validate its amount, frequency, duration, or safety. A fixed example also does not establish that everyone can tolerate it.
MOTS-c research includes experiments in cells and animals as well as measurements of the peptide made naturally in humans. Those are different kinds of evidence.
A 2021 Nature Communications paper measured naturally occurring MOTS-c around exercise in people and tested administered MOTS-c in mice. Its intermittent three-times-weekly treatment result was in mice. It does not validate the 5 mg human example above. Animal amounts in mg/kg should not be read as fixed milligram amounts for people. Read the original paper.
In its published compounding evaluation, FDA reported that it had not identified clinical studies or human exposure data for administered MOTS-c. That is a substantial limit on claims about a human dosing schedule or a predictable safety profile. Read FDA’s evaluation.
For the broader discussion of metabolism and the research background, see the MOTS-c metabolic health guide.
The amount in milligrams is different from the volume in milliliters. The same 5 mg amount can occupy different volumes depending on the concentration.
These examples assume the stated final volumes. They are calculations, not instructions to choose a liquid or prepare an injectable product.
| Vial amount and final volume | Concentration | Volume containing 5 mg | U-100 units |
|---|---|---|---|
| 10 mg in 1 mL | 10 mg/mL | 0.5 mL | 50 units |
| 10 mg in 2 mL | 5 mg/mL | 1 mL | 100 units |
| 20 mg in 2 mL | 10 mg/mL | 0.5 mL | 50 units |
The old 10 mg in 3 mL example produces about 3.33 mg/mL. At that concentration, 5 mg occupies 1.5 mL, or 150 U-100 units, which exceeds the capacity of a 1 mL syringe. Do not carry over an old unit count after changing the vial amount or volume.
Check other inputs with the peptide reconstitution calculator. The calculator checks arithmetic; it does not establish a suitable amount or preparation method.
A research vial’s purity result is not evidence that a particular human regimen is safe. Follow documented preparation and storage instructions for the actual product and intended laboratory use. A generic dosing chart cannot establish diluent compatibility, sterility, or the stability of a mixed solution.
This guide does not assign a universal storage period or a repeat-cycle break. Those details require evidence for the actual preparation and purpose. An absence of known problems is not proof of safety.
No. It is 5 mg three times per week, totaling 15 mg per week, over 10–12 weeks.
No. The example uses the same 5 mg amount throughout.
No. 5 mg equals 5,000 mcg. 500 mcg equals 0.5 mg.
No. Matching frequency alone does not establish a human amount, duration, benefit, or safety.
Reviewed October 23, 2026. Educational research reference only.
Joe Mars
Founder, The Peptide Report
I’m Joe Mars, and I’ve dedicated the past ten years to understanding peptide therapy, longevity, and how to optimize the body through practical, real-life testing. My journey started when I was tired, inflamed, and aging faster than I should have been. Clear information on peptides was almost impossible to find, so I dug in, researched nonstop, and tested protocols on myself.
Over the years, I have learned from experts like Jay Campbell, Dr. Seeds, Jim LaValle, and Ben Greenfield, and I have completely transformed my health. Now in my fifties, I feel stronger and sharper than I did in my twenties. That experience is why I write. I want to give people simple and honest guidance so they can use peptides safely and effectively.
I believe in data, smart protocols, and taking responsibility for your own health. You are the protocol. Your habits, your consistency, and your awareness shape your results. Through The Peptide Report, I share what actually works so you can make informed decisions and build a healthier, more resilient body.
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